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TOP2A Antibody [TPM2A-1]

TOP2A Antibody [TPM2A-1]

ArtNr	PRS-34-064-100ug
Hersteller	ProSci
Menge	100 ug
Kategorie	Oncology Antibodies By Cell Type Carcinoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, IF, IHC
Clon	TPM2A-1
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Konjugat/Tag	Unconjugated
NCBI	TOP2A
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	DNA topoisomerase 2-alpha,DNA topoisomerase II,alpha isozyme,TOP2A,TOP2
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer
Background	This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
By Type	Cancer
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1ug/10^6 cells IF: 1-2 ug/ml Western blot: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance. 1. Staining of FFPE tissue requires boiling sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Immunogen	Human partial recombinant protein was used as the immunogen for this TOP2A antibody.
Clonality	Monoclonal
NCBI Official Name	topoisomerase (DNA) II alpha 170kDa
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug

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Lieferung vsl. bis 04.12.2025

Express-Lieferung: 15 Tage sparen

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