Eotaxin Antibody [1.3_17H09-02C06]

Primary Antibodies

Eotaxin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚ C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚ C when reconstituted.

Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

None

This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 1.0-2.0 μ g/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μ g/mL.
Western Blot:
To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.