MIP-3 alpha Antibody [G3_4G10.3]

ELISA, WB, Neutralization Assay

MIP-3 alpha lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚ C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚ C when reconstituted.

Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It binds to CCR6.

Was product number XP-5713-M

None

This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μ g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 4.0-8.0 μ g/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with our biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ g/mL.
Western blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.