Eotaxin-3 Antibody [1.1_3H10-1D11]

Primary Antibodies, Functional Antibodies

Room Temp

Immunology, Chemokines & Cytokines, Functional Assays

None

This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μ g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 1.0-2.0 μ g/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μ g/mL.
Western Blot:
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.