FSH Protein (OPPA01020)

Hersteller AVIVA Systems Biology
Kategorie
Typ Proteins
Specific against other
Format The recombinant FSH was lyophilized from a concentrated (1 mg/ml) solution containing 0.4 mg sucrose 14.8 ug dibasic sodium phosphate and 6.42 ug monobasic sodium phosphate. Physical appearance: Sterile Filtered White lyophilized (freeze-dried)
Menge 2ug
Host Hamster
ArtNr OPPA01020
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Gene symbol
FSH
Protein name
Follitropin subunit beta
Molecular weight
38 kDa
Product format
Lyophilized PBS
Physical Appearance: Sterile filtered white freeze-dried powder
Gene id
2488
Reconstitution and storage
It is recommended to reconstitute the lyophilized Follicle Stimulating Hormone in sterile 18M-omega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Lyophilized recombinant FSH although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FSH should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Description of target
Follicle stimulating hormone (FSH) is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. FSH and LH act synergistically in reproduction: In women, in the ovary FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows it releases inhibin, which shuts off the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testes and is critical for spermatogenesis. In both males and females, FSH stimulates the maturation of germ cells. In females, FSH initiates follicular growth, specifically affecting granulosa cells. With the concomitant rise in inhibin B FSH levels then decline in the late follicular phase. This seems to be critical in selecting only the most advanced follicle to proceed to ovulation. At the end of the luteal phase, there is a slight rise in FSH that seems to be of importance to start the next ovulatory cycle. Like its partner, LH, FSH release at the pituitary gland is controlled by pulses of gonadotropin-releasing hormone (GnRH). Those pulses, in turn, are subject to the estrogen feed-back from the gonads. FSH Human Recombinant produced in HEK-293 cells is heterodimeric, glycosylated, polypeptide chain transfected with two expression plasmids encoding the human FSH-alpha chain (Accession # P01215) (Ala25-Ser116) and human FSH-beta chain (Asn19-Glu129) (Accession # P01225).
Purification
FSH human recombinant is purified by proprietary chromatographic techniques.
Biological activity
The ED50 as determined by cAMP accumulation in human FSH Receptor transfected Chinese Hamster Ovary cells was found to be 80-450 pg/ml.
Purity
Greater than 95.0% as determined by SDS-PAGE.

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Menge: 2ug
Lieferbar: In stock
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