Vergleich

Human Troponin T Type 3, Fast Skeletal (TNNT3) ELISA Kit

Hersteller Biomatik
Kategorie
Typ Elisa-Kit
Specific against Human
Format 96-Well Plate
Menge 96T
ArtNr BM-EKU07914
Voraussichtliche Lieferzeit 2-3 Weeks
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Estimated Turnaround Time
6-11 Business days
Target Name
Troponin T Type 3, Fast Skeletal
Target Synonyms
AMCD2B, DA2B, FSSV, fTnT, Troponin T3, Beta-TnTF, Fast skeletal muscle troponin T
Sample Type
Serum, Plasma, Tissue homogenates and other biological fluids
Detection Range
15.6-1000 pg/mL
Sensitivity
6.1 pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 3, Fast Skeletal were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 3, Fast Skeletal were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
Detection Method
Colorimetric
Assay Time
3 hours
Assay Type
Double-antibody Sandwich
Shipping Condition
Ice packs
Storage
Short term: 4C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date
8 months
UniProt ID
P45378
Specificity
This assay has high sensitivity and excellent specificity for detection of Troponin T Type 3, Fast Skeletal
Cross Activity
No significant cross-reactivity or interference between Troponin T Type 3, Fast Skeletal and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Troponin T Type 3, Fast Skeletal and analogues was observed.
Assay Procedure
1.Prepare all reagents, samples and standards; 2.Add 100 uL standard or sample to each well. Incubate 2 hours at 37C; 3.Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37C; 4.Aspirate and wash 3 times; 5.Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37C; 6.Aspirate and wash 5 times; 7.Add 90 uL Substrate Solution. Incubate 10-20 minutes at 37C; 8.Add 50 uL Stop Solution. Read at 450 nm immediately.
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For research use only. Not for diagnostic procedures.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance
Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.
Research Area
Cardiovascular biology

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Menge: 96T
Lieferbar: In stock
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