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Mouse Vinculin (VCL) ELISA Kit

Hersteller Biomatik
Kategorie
Typ Elisa-Kit
Specific against Mouse
Format 96 well plate
Applikationen ELISA
Menge 96T
ArtNr BM-EKU08146
Targets VCL
Voraussichtliche Lieferzeit 2-3 Weeks
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Detection Range
0.312-20 ng/mL
Sensitivity
0.128 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Assay Time
3 hours
UniProt ID
Q64727
Gene ID
22330
Gene Name
Vinculin
Gene Aliases
MVCL; Metavinculin
Assay Type
Sandwich
Detection Method
Colorimetric
Shipping Condition
Blue ice
Storage Instructions
-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date
8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of Vinculin (VCL). No significant cross-reactivity or interference between Vinculin (VCL) and analogues was observed.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vinculin (VCL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vinculin (VCL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vinculin (VCL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vinculin (VCL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vinculin (VCL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vinculin (VCL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For research use only. Not for diagnostic procedures.
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Developmental science;

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Menge: 96T
Lieferbar: In stock
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