Anti-FTO Antibody

Primary Antibodies

58282 MW

WB: 1:500-1:2, 000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Alpha-ketoglutarate-dependent dioxygenase FTO

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Dioxygenase that repairs alkylated DNA and RNA by
oxidative demethylation. Has highest activity towards single-
stranded RNA containing 3-methyluracil, followed by single-
stranded DNA containing 3-methylthymine. Has low demethylase
activity towards single-stranded DNA containing 1-methyladenine or
3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically
demethylates N (6)-methyladenosine (m6A) RNA, the most prevalent
internal modification of messenger RNA (mRNA) in higher eukaryotes
(PubMed:22002720, PubMed:26458103). Has no activity towards 1-
methylguanine. Has no detectable activity towards double-stranded
DNA. Requires molecular oxygen, alpha-ketoglutarate and iron.
Contributes to the regulation of the global metabolic rate, energy
expenditure and energy homeostasis. Contributes to the regulation
of body size and body fat accumulation (PubMed:18775698,
PubMed:20376003). In particular, it is involved in the regulation
of thermogenesis and the control of adipocyte differentiation into
brown or white fat cells (PubMed:26287746).

Boster Bio Anti-FTO Antibody catalog # A00219-1. Tested in WB, ICC/IF, IHC applications. This antibody reacts with Human.