Anti-FTO Antibody

ArtNr	BOS-A00219-2
Hersteller	Boster
Menge	100 ul
Quantity options	0.1 mg 100 ul 100 ul
Kategorie	Metabolism Diabetes Glucose Homeostasis Obesity Immunohistochemistry Western Blot ELISA Immunofluorescence Immunostaining
Format	Liquid
Applikationen	WB, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Alias	Alpha-ketoglutarate-dependent dioxygenase FTO,1.14.11.-
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Molecular Weight	58282 MW
Clonality	Polyclonal
Application Details	WB 1:500-1:2000 ELISA 1:20000
Gene Name	FTO
Gene Full Name	Alpha-ketoglutarate-dependent dioxygenase FTO
Immunogen	The antiserum was produced against synthesized peptide derived from human FTO. AA range:19-68
Contents	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Protein Name	Alpha-ketoglutarate-dependent dioxygenase FTO
Protein Function	Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N (6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).
Subcellular Localization	Nucleus
Description	Boster Bio Anti-FTO Antibody catalog # A00219-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
Short Description	Boster Bio Anti-FTO Antibody catalog # A00219-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

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BOS-A00219-2-datasheet.pdf

BOS-A00219-2-safety-datasheet.pdf