Anti-LPL Antibody (Center)

ArtNr	BOS-A00316-1-80ul
Hersteller	Boster
Menge	80 ul
Quantity options	400 ul 80 ul
Kategorie	Metabolism Cholesterol & Lipid Metabolism Flow Cytometry Immunohistochemistry Western Blot Enzymes Fatty Acid Catabolism
Applikationen	WB, FC, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Alias	Lipoprotein lipase,LPL,LPL,LIPD
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Manufacturer - Category	Primary Antibodies
Storage Conditions	Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight	53162 Da
Clonality	Polyclonal
Application Details	WB: 1:1000-1:2000 IHC-P: 1:10-1:50 FC: 1:25
Gene Name	LPL
Background	LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq].
Immunogen	This LPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-327 amino acids from the Central region of human LPL.
Contents	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Manufacturer - Research Category	Cancer, Cardiovascular, Metabolism, Signal Transduction
Protein Function	Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed:8675619, PubMed:11342582, PubMed:27578112). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed:7592706, PubMed:12032167). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed:24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed:11342582, PubMed:27811232).
Subcellular Localization	Cell membrane {ECO:0000250\|UniProtKB:P11151}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P11151}; Extracellular side {ECO:0000250\|UniProtKB:P11151}. Secreted. Secreted, extracellular space, extracellular matrix. Note=Newly synthesized LPL binds to cell surface heparan proteoglycans and is then released by heparanase Subsequently, it becomes attached to heparan proteoglycan on endothelial cells (PubMed:27811232). Locates to the plasma membrane of microvilli of hepatocytes with triglyceride-rich lipoproteins (TRL) Some of the bound LPL is then internalized and located inside non- coated endocytic vesicles (By similarity) {ECO:0000250\|UniProtKB:P11151, ECO:0000269\|PubMed:27811232}
Description	Boster Bio Anti-LPL Antibody (Center) (Catalog # A00316-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Tissue Specificity	Detected in blood plasma (PubMed:2340307, PubMed:11893776, PubMed:12641539). Detected in milk (at protein level) (PubMed:2340307).
Predicted Reactivity	Bovine, Mouse, Pig, Rat, Sheep

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