Anti-ERCC2 Antibody

Primary Antibodies

86909 MW

WB: 1:500-1:2000
ICC/IF: 1:50-1:100

General transcription and DNA repair factor IIH helicase subunit XPD

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

ATP-dependent 5'-3' DNA helicase, component of the
general transcription and DNA repair factor IIH (TFIIH) core
complex, which is involved in general and transcription-coupled
nucleotide excision repair (NER) of damaged DNA and, when
complexed to CAK, in RNA transcription by RNA polymerase II. In
NER, TFIIH acts by opening DNA around the lesion to allow the
excision of the damaged oligonucleotide and its replacement by a
new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2
is required for DNA opening. In transcription, TFIIH has an
essential role in transcription initiation. When the pre-
initiation complex (PIC) has been established, TFIIH is required
for promoter opening and promoter escape. Phosphorylation of the
C-terminal tail (CTD) of the largest subunit of RNA polymerase II
by the kinase module CAK controls the initiation of transcription.
XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH
complex. Involved in the regulation of vitamin-D receptor
activity. As part of the mitotic spindle-associated MMXD complex
it plays a role in chromosome segregation. Might have a role in
aging process and could play a causative role in the generation of
skin cancers.

Boster Bio Anti-ERCC2 Antibody catalog # A00694. Tested in WB, ICC/IF applications. This antibody reacts with Human, Rat.