Anti-PKC-pan ( Alpha T497) PRKCA Antibody

Primary Antibodies

76750 MW

WB: 1:500-1:1000
IHC: 1:50-1:200

Protein kinase C alpha type

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1 mg/ml

Calcium-activated, phospholipid- and diacylglycerol
(DAG)-dependent serine/threonine-protein kinase that is involved
in positive and negative regulation of cell proliferation,
apoptosis, differentiation, migration and adhesion, tumorigenesis,
cardiac hypertrophy, angiogenesis, platelet function and
inflammation, by directly phosphorylating targets such as RAF1,
BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving
MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and
cell growth arrest by positive and negative regulation of the cell
cycle. Can promote cell growth by phosphorylating and activating
RAF1, which mediates the activation of the MAPK/ERK signaling
cascade, and/or by up-regulating CDKN1A, which facilitates active
cyclin-dependent kinase (CDK) complex formation in glioma cells.
In intestinal cells stimulated by the phorbol ester PMA, can
trigger a cell cycle arrest program which is associated with the
accumulation of the hyper-phosphorylated growth-suppressive form
of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B.
Exhibits anti-apoptotic function in glioma cells and protects them
from apoptosis by suppressing the p53/TP53-mediated activation of
IGFBP3, and in leukemia cells mediates anti-apoptotic action by
phosphorylating BCL2. During macrophage differentiation induced by
macrophage colony-stimulating factor (CSF1), is translocated to
the nucleus and is associated with macrophage development. After
wounding, translocates from focal contacts to lamellipodia and
participates in the modulation of desmosomal adhesion. Plays a
role in cell motility by phosphorylating CSPG4, which induces
association of CSPG4 with extensive lamellipodia at the cell
periphery and polarization of the cell accompanied by increases in
cell motility. During chemokine-induced CD4 (+) T cell migration,
phosphorylates CDC42-guanine exchange factor DOCK8 resulting in
its dissociation from LRCH1 and the activation of GTPase CDC42
(PubMed:28028151). Is highly expressed in a number of cancer cells
where it can act as a tumor promoter and is implicated in
malignant phenotypes of several tumors such as gliomas and breast
cancers. Negatively regulates myocardial contractility and
positively regulates angiogenesis, platelet aggregation and
thrombus formation in arteries. Mediates hypertrophic growth of
neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-
dependent signaling pathway, and upon PMA treatment, is required
to induce cardiomyocyte hypertrophy up to heart failure and death,
by increasing protein synthesis, protein-DNA ratio and cell
surface area. Regulates cardiomyocyte function by phosphorylating
cardiac troponin T (TNNT2/CTNT), which induces significant
reduction in actomyosin ATPase activity, myofilament calcium
sensitivity and myocardial contractility. In angiogenesis, is
required for full endothelial cell migration, adhesion to
vitronectin (VTN), and vascular endothelial growth factor A
(VEGFA)-dependent regulation of kinase activation and vascular
tube formation. Involved in the stabilization of VEGFA mRNA at
post-transcriptional level and mediates VEGFA-induced cell
proliferation. In the regulation of calcium-induced platelet
aggregation, mediates signals from the CD36/GP4 receptor for
granule release, and activates the integrin heterodimer ITGA2B-
ITGB3 through the RAP1GAP pathway for adhesion. During response to
lipopolysaccharides (LPS), may regulate selective LPS-induced
macrophage functions involved in host defense and inflammation.
But in some inflammatory responses, may negatively regulate NF-
kappa-B-induced genes, through IL1A-dependent induction of NF-
kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-
tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1,
which modulates EIF4G1 binding to MKNK1 and may be involved in the
regulation of EIF4E phosphorylation. Phosphorylates KIT, leading
to inhibition of KIT activity. Phosphorylates ATF2 which promotes
cooperation between ATF2 and JUN, activating transcription.

Boster Bio Anti-PKC-pan ( Alpha T497) PRKCA Antibody catalog # A00743T497. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.