Antibodies

Anti-HLA-DQB1/2 Antibody

Hersteller Boster
Kategorie
Typ Antibody
Specific against other
Applikationen WB, IHC
Menge 100ul
Host Rabbit
ArtNr BOS-A00106
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Gene name
HLA-DQB1
Clonality
Polyclonal
Conjugate
No
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Description
Polyclonal antibody for HLA DQB1/HLA-DQB1 detection. Host: Rabbit.Size: 100ul. Tested applications: IHC. Reactive species: Human. HLA DQB1/HLA-DQB1 information: Molecular Weight: 29991 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
Uniprot ID
P01920
Immunogen
Synthesized peptide derived from the Internal region of human HLA-DQB1/2.
Purification
Immunogen affinity purified
Storage
Store at -20° C for one year, at 4° C for one month. We suggest the antibody be aliquotted into small vials and stored frozen at -20° C upon receiving. Avoid repeated freezing and thawing.
Application details
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Reactivity
Human
Research category
|immunology|adaptive immunity|mhc|class ii
Synonyms
HLA class II histocompatibility antigen, DQ beta 1 chain, MHC class II antigen DQB1, HLA-DQB1, HLA-DQB,
Molecular weight
29991 MW
Protein function
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Menge: 100ul
Lieferbar: Out of stock
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