Antibodies

Anti-DNA2/Dna2LL Antibody

Hersteller Boster
Kategorie
Typ Antibody
Specific against other
Applikationen WB
Menge 100ul
Host Rabbit
ArtNr BOS-A01526
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Gene name
DNA2
Clonality
Polyclonal
Conjugate
No
Contents
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description
Polyclonal antibody for DNA2L/DNA2 detection. Host: Rabbit.Size: 100ul. Tested applications: WB. Reactive species: Human. DNA2L/DNA2 information: Molecular Weight: 120415 MW; Subcellular Localization: Nucleus. Mitochondrion. Was initially reported to be exclusively mitochondrial (PubMed:18995831). However, it was later shown to localize both in mitochondrion and nucleus (PubMed:19487465).
Uniprot ID
P51530
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human DNA2L
Purification
Immunogen Affinity Purified
Storage
At -20° C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20° C for a longer time.Avoid repeated freezing and thawing.
Application details
WB: Dilution:0.2-1ug/ml
Reactivity
Human, Mouse, Rat
Predicted reactivity
Bovine, Canine, Equine, Guinea Pig, Pig, Rabbit
Research category
|epigenetics and nuclear signaling|dna / rna|dna synthesis
Synonyms
DNA replication ATP-dependent helicase/nuclease DNA2, hDNA2, DNA replication ATP-dependent helicase-like homolog, DNA replication nuclease DNA2, 3.1.-.-, DNA replication ATP-dependent helicase DNA2, 3.6.4.12, DNA2, DNA2L, KIAA0083,
Molecular weight
120415 MW
Protein function
Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. .

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Menge: 100ul
Lieferbar: Out of stock
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