Anti-Amphiregulin/Areg Antibody

Hersteller Boster
Kategorie
Typ Antibody Primary
Specific against other
Isotype IgG
Format Lyophilized
Applikationen WB, IHC, ELISA
Menge 100ug/vial
Host Rabbit
ArtNr BOS-A01787-1
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Clonality
Polyclonal
Description short
Rabbit IgG polyclonal antibody for Amphiregulin detection. Tested with WB, IHC-P, ELISA(Cap) in Mouse, Rat.
Specificity
No cross reactivity with other proteins.
Reactivity
Reacts with: mouse, rat
Application details
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Paraffin-embedded Section)|0.5-1μ g/ml
ELISA(Cap)|1-5μ g/ml
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E. coli-derived mouse Amphiregulin recombinant protein (Position:V100-K191).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Background
Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor (EGF) family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. It is mapped to 9q32. It has been shown to play a role in immunity, inflammation, tissue repair, and lung and mammary gland development. Homozygous knockout mice for this gene exhibit impaired immune system regulation in the skin and gene expression changes characteristic of chronic liver damage.
Reference
1. Ciarloni, L., Mallepell, S., Brisken, C. Amphiregulin is an essential mediator of estrogen receptor alpha function in mammary gland development. Proc. Nat. Acad. Sci. 104: 5455-5460, 2007.
2. Cook, P. W., Piepkorn, M., Clegg, C. H., Plowman, G. D., DeMay, J. M., Brown, J. R., Pittelkow, M. R. Transgenic expression of the human amphiregulin gene induces a psoriasis-like phenotype. J. Clin. Invest. 100: 2286-2294, 1997.
Gene Name
Areg
Protein Name
Amphiregulin
Gene Full Name
amphiregulin
Synonyms
Amphiregulin; AR; Schwannoma-derived growth factor; SDGF; Areg; Sdgf
Uniprot ID
P31955
Entrez GeneID
11839
Menge: 100ug/vial
Lieferbar: In stock
Listenpreis: 280,01 €
Preis: 280,01 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen