Anti-L1/ORF2 Antibody

Boster Bio Anti-L1/ORF2 Antibody (Catalog # A19762). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Chimpanzee.

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.

Polyclonal

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

ELISA: 1:10, 000
WB: 1:2, 000-1_10, 000

This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH).  The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein.  This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.   Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays.   A BLAST analysis was used to suggest cross-reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.

Putative Dihydrofolate reductase

Liquid (sterile filtered)