Mouse RANK/CD265 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

66621 MW

Tumor necrosis factor receptor superfamily member 11A

Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.

Adapters, Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Immunology, Innate Immunity, Signal Transduction, Structures, TNF Superfamily, Transmembrane, Vascular Inflammation

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.

Mouse RANK/CD265 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Tnfrsf11a in cell culture supernatants and serum.

cell culture supernatants and serum.

Catalog number | Description | Quantity | Storage of opened/reconstituted material
EZ0830-CA | Rat anti-mouse RANK/CD265 monoclonal antibody (Capture Antibody) | 500 µ l, 4 µ g/mL
(recommended dilution 1:100)
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0830-DA | Biotinylated goat anti-mouse RANK/CD265 polyclonal antibody (Detection Antibody) | 500 µ l (recommended dilution 1:100)
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µ l (recommended dilution 1:100)
EZ0830-ST | Lyophilized recombinant mouse RANK/CD265 standard | 10 ng/tubex3
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit.

There is no detectable cross-reactivity with other relevant proteins.