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Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit

Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK0481
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Metabolism Glucose Homeostasis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<5 pg/ml
Sequence	Contains 2 death domains.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Tumor necrosis factor receptor superfamily member 11B,Osteoclastogenesis inhibitory factor,Osteoprotegerin,Tnfrsf11b,Ocif,Opg
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	45923 MW
Gene Name	Tnfrsf11b
Gene Full Name	Tumor necrosis factor receptor superfamily member 11B
Manufacturer - Specificity	Natural and recombinant mouse OPG
Background	Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.
Immunogen	Expression system for standard: NS0; Immunogen sequence: E22-L401
Protein Name	Tumor necrosis factor receptor superfamily member 11B
Protein Function	Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Subcellular Localization	Secreted.
Description	Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Tnfrsf11b in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.
Sample Type	cell culture supernatants, serum and plasma (EDTA).
Sequence Similarities	Contains 2 death domains.
Sample Data	Concentration (pg/ml) \| 0 \| 93.7 \| 187.5 \| 375 \| 750 \| 13000 \| 3000 \| 6000 O.D. \| 0.000 \| 0.082 \| 0.173 \| 0.294 \| 0.522 \| 0.976 \| 1.585 \| 2.118
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 133 \| 800 \| 3535 \| 139 \| 767 \| 3643 Standard deviation \| 5.85 \| 54.4 \| 187.35 \| 7.3%9 \| 55.3%9 \| 225.86 CV (%) \| 4.4% \| 6.8% \| 5.3% \| 4.6% \| 7.3% \| 6.2%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 133 \| 157 \| 157 \| 146 \| 148 \| 9.88 \| 6.6% Sample 2 \| 800 \| 766 \| 743 \| 710 \| 754 \| 32.84 \| 4.3% Sample 3 \| 3535 \| 3949 \| 3581 \| 3584 \| 3662 \| 166.69 \| 4.5% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from rat\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	10 ng/tube
Anticoagulant	EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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