Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit

Quick ELISA Kits

102451 MW

Interleukin-6 receptor subunit beta

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Interleukin-6 receptor subunit beta

Membrane; Single-pass type I membrane protein.

Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 159 | 1404 | 4588 | 158 | 1473 | 4382
Standard deviation | 11.6 | 71.6 | 339.51 | 14.06 | 81.01 | 333.03
CV (%) | 7.3% | 7.6%% | 7.4% | 8.9% | 5.5% | 7.6%

monoclonal antibody from rat|polyclonal antibody from goat

10 ng/tube

Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.