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Human Resistin PicoKine® Quick ELISA Kit

Human Resistin PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK0581
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<3 pg/ml
Sequence	Belongs to the resistin/FIZZ family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Resistin,Adipose tissue-specific secretory factor,ADSF,C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein,Cysteine-rich secreted protein A12-alpha-like 2,Cysteine-rich secreted protein FIZZ3,RETN,FIZZ3,HXCP1,RSTN,UNQ407/PRO1199
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	11419 MW
Gene Name	RETN
Gene Full Name	Resistin
Manufacturer - Specificity	Natural and recombinant human Resistin
Background	Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: K19-P108
Protein Name	Resistin
Protein Function	Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Subcellular Localization	Secreted.
Description	Human Resistin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Expressed only in fatty tissues.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the resistin/FIZZ family.
Sample Data	Concentration (pg/ml) \| 0 \| 78 \| 156 \| 312 \| 625 \| 1250 \| 2500 \| 5000 O.D. \| 0.013 \| 0.169 \| 0.306 \| 0.483 \| 0.926 \| 1.391 \| 1.920 \| 2.305
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 135 \| 639 \| 2067 \| 142 \| 636 \| 2227 Standard deviation \| 8.1 \| 40.25 \| 161.22 \| 8.52 \| 46.42 \| 177.83 CV (%) \| 6% \| 7.3% \| 7.8% \| 6% \| 7.3% \| 7.9%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 135 \| 137 \| 143 \| 146 \| 140 \| 4.43 \| 3.1% Sample 2 \| 639 \| 653 \| 634 \| 737 \| 665 \| 41.72 \| 6.2% Sample 3 \| 2067 \| 2270 \| 2337 \| 2337 \| 2252 \| 110.67 \| 4.9% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Alias	Resistin, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, RETN, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-FEK0581-datasheet.pdf

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