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Human RBP4/Retinol Binding Protein 4 PicoKine® Quick ELISA Kit

Human RBP4/Retinol Binding Protein 4 PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK0831
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Metabolism Diabetes Glucose Homeostasis Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
Sequence	Belongs to the calycin superfamily. Lipocalin family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Retinol-binding protein 4,Plasma retinol-binding protein,PRBP,RBP,Plasma retinol-binding protein (1-182),Plasma retinol-binding protein (1-181),Plasma retinol-binding protein (1-179),Plasma retinol-binding protein (1-176),RBP4,PRO2222
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	23010 MW
Gene Name	RBP4
Gene Full Name	Retinol-binding protein 4
Manufacturer - Specificity	Natural and recombinant human RBP4
Background	Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.
Immunogen	Expression system for standard: NS0; Immunogen sequence: E19-L201
Protein Name	Retinol-binding protein 4
Protein Function	Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
Subcellular Localization	Secreted.
Description	Human RBP4/Retinol Binding Protein 4 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human RBP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Sequence Similarities	Belongs to the calycin superfamily. Lipocalin family.
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 200000000 \| 20000000 \| 2000000 \| 200000 \| 20000 O.D. \| 0.029 \| 0.066 \| 0.108 \| 0.215 \| 0.495 \| 1.074 \| 1.866 \| 2.408
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 621 \| 2874 \| 9419 \| 643 \| 2778 \| 10172 Standard deviation \| 44.09 \| 227.04 \| 668.74 \| 53.36 \| 247.24 \| 752.72 CV (%) \| 7.1% \| 7.9% \| 7.1% \| 8.3% \| 8.9% \| 7.4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 621 \| 574 \| 637 \| 603 \| 608 \| 23.39 \| 3.8% Sample 2 \| 2874 \| 2703 \| 2656 \| 2896 \| 2782 \| 104.37 \| 3.7% Sample 3 \| 9419 \| 8739 \| 8492 \| 8429 \| 8769 \| 392.34 \| 4.4% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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