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Rat CD44 PicoKine® Quick ELISA Kit

Rat CD44 PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK1417
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Oncology By Cell Type Carcinoma Cell Biology Cell Movement Cell Adhesion ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Colorectal Cancer Breast Cancer Head & Neck Cancer Testicular & Prostate Cancer Gastric Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	<10 pg/ml
Sequence	Contains 1 Link domain.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD44 antigen,Extracellular matrix receptor III,ECMR-III,GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1,PGP-1,Phagocytic glycoprotein I,PGP-I,CD44,Cd44
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	55946 MW
Gene Name	Cd44
Gene Full Name	CD44 antigen
Manufacturer - Specificity	Natural and recombinant rat CD44
Background	CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q22-E271
Protein Name	CD44 antigen
Protein Function	Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function.
Subcellular Localization	Cell membrane; Single-pass type I membrane protein. Colocalizes with actin in membrane protrusions at wounding edges.
Description	Rat CD44 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Cd44 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Contains 1 Link domain.
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1250 \| 2500 \| 5000 \| 10000 \| 20000 O.D. \| 0.035 \| 0.163 \| 0.274 \| 0.458 \| 0.861 \| 1.249 \| 1.832 \| 2.254
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 481 \| 1665 \| 7855 \| 452 \| 1537 \| 7710 Standard deviation \| 28.37 \| 129.87 \| 439.88 \| 32.54 \| 136.79 \| 516.57 CV (%) \| 5.6% \| 7.8% \| 5.6% \| 7.2% \| 8.9% \| 6.7%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 481 \| 494 \| 514 \| 475 \| 491 \| 14.94 \| 3% Sample 2 \| 1665 \| 1566 \| 1588 \| 1645 \| 1616 \| 40.39 \| 2.4% Sample 3 \| 7855 \| 9238 \| 8886 \| 9256 \| 8808 \| 570.06 \| 6.4% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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