Antibodies

Mouse Anti-Human CXCR2 Clone 19 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon Clone19
Isotype IgG1
Applikationen WB, FC, ELISA
Menge 0.5 mg
Host Mouse
ArtNr CS-CMC204
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Flow cytometry, Western blot, ELISA, Immunohistochemistry. Suitable for Flow Cytometry on isolated human neutrophils and on cells transfected with CXCR2.
Description
CXCR1 and CXCR2, previously known as IL-8RA, or type I IL-8 receptor, and IL-8RB, or type II IL-8 receptor, respectively, have been shown to share approximately 77% amino acid sequence identity. IL8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca2+ levels. While IL-8 and Granulocyte Chemotactic Protein 2 (GCP2) have been shown to exert their biological activity by binding to both receptors, GRO-alpha, GRO-beta, GRO-gamma, NAP2 and ENA-78 bind only to CXCR2. CXCR1 and CXCR2 are expressed on neutrophils and mast cells, but not on B- or T-lymphocytes. In cells transfected with either CXCR1 or CXCR2, clone 19 showed no cross-reactivity with CXCR1.
Formulation
Purified immunoglobulin in PBS, pH 7.2, without preservative, 0.22 micron sterile filtered
Immunogen
19 amino acid peptide corresponding to aa 1-19 at the N-terminus of human CXCR2
Purification
Purified from ascites by Protein G chromatography.
Storage and Stability
Centrifuge vial prior to opening. Store at 2-4C. For long term storage, aliquot and store at -20C. Avoid repeated freeze-thaw cycles.
Concentration
1.0 mg/ml
Cross-Reactivity
In cells transfected with either CXCR1 or CXCR2, clone 19 showed no cross-reactivity with CXCR1.
Application Notes
Flow Cytometry: use 0.5-1.0 ug per 106 cells.Neutralization: incubation of human neutrophils with 2.0 ug/ml of clone 19 completely blocks IL-8-mediated chemotaxis. The optimal concentration should be determined by the user for each specific application.
Menge: 0.5 mg
Lieferbar: Out of stock
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