Mouse Anti-Human DFF45 Clone 6B8SG Affinity Purified mAb

For Western Blot, use a working dilution of 0.5-4 ug/ml.The optimal concentration should be determined by the user for each specific application.

Mouse Anti-Human DFF45 Clone 6B8SG Affinity Purified monoclonal antibody. During apoptosis, various stimuli activate a protease cascade of caspases that eventually cause degradation of chromosomal DNA. DFF45 (DNA fragmentation factor 45) is a downstream regulator of DNA degradation (DFF40). DFF40 is an apoptotic DNase with a high specific activity that exists in an inactive complex with DFF45 in living cells. Cleavage of DFF45, which is mediated by caspase-3, leads to DFF40 release where it then enters nucleus to function as a nuclease. DFF45 may inhibit the DNAse activity of DFF40 and its nuclear translocation. Activation of DFF40 causes DNA degradation, a hallmark of apoptosis.

Recombinant human DFF45

Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.