Antibodies

Mouse Anti-Human Hsp70 Clone S5 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon S5
Isotype IgG1
Applikationen WB, IHC
Menge 100 ug
Host Mouse
ArtNr CS-CMH115
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot Immunoprecipitation Immunohistochemistry (Frozen and Paraffin) FACS Analysis
Description
Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP, in vitro , but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus, b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus, c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum, and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell.
Formulation
Liquid in PBS + 1 mg/ml BSA + 1.5 mM sodium azide + 50% glycerol. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73).
Immunogen
Recombinant human Hsp70
Reconstitution
The antibody solution should be gently mixed before use. The optimal concentration should be determined by the user for each specific application.
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.5 mg/ml
Cross-Reactivity
Reacts with human, mouse, rat, monkey, cow, rabbit, hamster, guinea pig, dog, sheep, chicken, pig. Does not cross-react with the constitutive HSPA8.
Application Notes
Western blot analysis (1 ug/ml), immunoprecipitation (5-10 ug/ml), Immunohistochemistry (5-20 ug/ml, frozen & paraffin), and FACS analysis. However, the optimal conditions should be determined individually. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73).
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 780,52 €
Preis: 780,52 €
lieferbar

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