Rabbit Anti-Human CALNA/PPP3CA Affinity Purified pAb

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Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPC133
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot Immunoprecipitation
Description
Rabbit Anti-Human Calcineurin A Affinity Purified polyclonal antibody. Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death.
Formulation
Liquid in PBS + pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots.
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 267 of human Calcineurin A
Purification
Biospecific affinity chromatography
Reconstitution
The antibody solution should be gently mixed before use.
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.2 mg/ml
Cross-Reactivity
Human, mouse, rat, rabbit, bovine, hamster, and dog
Application Notes
Western Blotting (1-4 ug/ml) and for immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A..

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Menge: 100 ug
Lieferbar: In stock
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