Rabbit Anti-Human PGF aa1-20 pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB, ICC, ELISA
Menge 200 ug
Host Rabbit
ArtNr CS-CPP500B
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot: We recommend using the IgG at a concentration of 10 ug/ml in Western Blot analysis. It will detect 25-50 ng/lane of recombinant human PGF-1 and PGF-2 under reducing conditions. Dimers are detected at higher protein amounts/lane. ELISA: To detect human PlGF by direct ELISA a concentration of at least 1-5 ug/ml is required. This purified IgG, in combination with compatible secondary reagents, allows the detection of 1.0- 2.5 ng/well of recombinant human PGF-1 and PGF-2. Immunocytochemistry: To detect human PGF in tissue section (e.g. placenta tissue) use 5-25 ug/ml IgG. This antibody, in combination with compatible secondary reagents, allows the detection of PGF protein in different human tissue including placenta and brain tumors. Note: The optimal concentration should be determined by the user for each specific application.
Description
Rabbit Anti-Human PGF aa1-20 pAb is produced from sera of rabbits immunized with a highly purified N-terminal peptide of human PGF (placenta growth factor). Anti-PlGF was purified by affinity chromatography with immobilized Protein A. Placenta growth factor (PGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PGF1), 152 (PGF2), and 203 (PGF3) amino acids (aa) respectively. Only PGF2 contains a highly basic heparin binding 21 aa insert at the C-terminus. In the mouse, only one PGF that is the equivalent of human PGF2 has been identified. Human PGF1 shares 56%, 55%, 74% and 95% aa identity with the appropriate isoform of mouse, rat, canine and equine PGF. PGF is mainly found as variably glycosylated, secreted, 55-60 kDa disulfide linked homodimers. Mammalian cells expressing PGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PGF increases during pregnancy, reaching a peak in mid-gestation;, this increase is attenuated in preeclampsia. However, deletion of PGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PGF show impaired angiogenesis in response to ischemia. PGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PGF and VEGF therefore compete for binding to VEGF R1, allowing high PGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PGF (especially PGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PGF2, but not PGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.
Formulation
Lyophilized from PBS, pH 7.4, without preservatives.
Specificity
Human
Immunogen
N-terminal 20 amino acid peptide of Human PGF
Purification
Protein A chromatography
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of >0.5 mg/ml.
Storage and Stability
Stable at 2-4C for one month and over a year when kept at -20C. After reconstitution, stable for at least six weeks at 2-4C. Avoid repeated freeze-thaw cycles.
Cross-Reactivity
The antibody will react with all human PGF isoforms. Cross-reactivity with other species is not investigated.
Menge: 200 ug
Lieferbar: In stock
Listenpreis: 428,31 €
Preis: 428,31 €
lieferbar

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Salesmanager: Arne Pelz

Email: sales@hoelzel.de

Arbeitsanleitung

CS-CPP500B.pdf