Rabbit Anti-Human Placenta Growth Factor pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB, ELISA
Menge 200 ug
Host Rabbit
ArtNr CS-CPP501B
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot: Use 2-5 ug/ml ELISA: Use at 1-5 ug/ml The optimal concentration should be determined by the user for each specific application.
Description
Rabbit Anti-Human PGF polyclonal antibody. Human Placenta Growth Factor-2 (PGF-2), a 22 kDa protein consisting of 152 amino acid residues, is produced as a homodimer. PGF is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PGF acts only as a weak mitogen for those cell types possessing receptors for binding (e.g. vascular endothelial cells). At least one high-affinity receptor for PGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human uMbilical vein endothelial cells and monocytes). In addition to its action as a weak mitogen it is also a chemoattractant for monocytes and endothelial cells. Two different proteins are generated by differential splicing of the human PGF gene: PGF-1 (131 aa native chain) and PGF-2 (152 aa native chain). Both mitogens are secretable proteins, but PGF-2 can bind to heparin with high affinity. PGF is apparently a homodimer, but preparations of PGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms possess similar biological activities. If PGF is angiogenic, in vivo, is not clear. However, heterodimers between VEGF and PGF are mitogenic for endothelial cells and have strong angiogenic activity, in vivo, (e.g. in the CAM assay or in the cornea pocket assay). Different cells and tissues (e.g. placenta) express PGF-1 and PGF-2 at different rates. A much related protein of PGF is VEGF with about 53% homology and VEGF-B with similar biological activities.
Formulation
Lyophilized from PBS solution
Specificity
Human PGF
Immunogen
Recombinant Human PGF-2 (Leu19-Arg170) expressed in insect cells (Cell Sciences Cat # CRP202)
Purification
Protein A chromatography
Reconstitution
Centrifuge vial prior to opening. Reconstitute with sterile water to a concentration of 0.1-1.0 mg/ml.
Storage and Stability
The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze/thaw cycles.
Cross-Reactivity
Reacts with all human PGF isoforms. Cross-reactivity to other species has not been determined.
Menge: 200 ug
Lieferbar: In stock
Listenpreis: 543,94 €
Preis: 543,94 €
lieferbar

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