Antibodies

Rabbit Anti-Human RAIDD Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPR105
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot
Description
Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved, in vivo, by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
Formulation
100 ug (0.2 mg/ml) affinity purified rabbit anti-RAIDD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol + 0.5% BSA + 0.01% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Detects a 22 kDa band, corresponding to the expected MW = RAIDD on immunoblots
Immunogen
Synthetic peptide surrounding amino acid 184 of human RAIDD
Purification
Affinity purified
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.2 mg/ml
Application Notes
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 22 kDa band, corresponding to the expected molecular mass of RAIDD on immunoblots. The optimal concentration should be determined by the user for each specific application.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 659,19 €
Preis: 659,19 €
lieferbar

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