Rabbit Anti-Human RAIDD Affinity Purified pAb

Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 22 kDa band, corresponding to the expected molecular mass of RAIDD on immunoblots. The optimal concentration should be determined by the user for each specific application.

Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved, in vivo, by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.

Synthetic peptide surrounding amino acid 184 of human RAIDD

Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.