Rabbit Anti-Human RAF1 Affinity Purified pAb

Hersteller Cell Sciences
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPR106
eClass 6.1 32160702
eClass 9.0 32160702
Western Blot Immunoprecipitation Immunohistochemistry
Rabbit Anti-Human RAF1 Affinity Purified polyclonal antibody. Human RAF1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of MEK1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (MEK1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. RAF1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in RAF1. RAF1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. RAF1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2), and the catalytic domain (CR3). RAF1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins, and the molecular chaperones hsp90 and p50.
100 ug (0.2 mg/ml) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol + 1% BSA + 0.02% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Recognizes a 74 kDa RAF1 protein on SDS-PAGE immunoblots
Synthetic peptide mapping to the C-terminus of human RAF1
Affinity purified.
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
0.2 mg/ml
Cross reacts with mouse and rat RAF1. Does not cross react with Raf-A and Raf-B.
Application Notes
Western blot analysis (1-4 ug/ml), immunoprecipitation (15-25 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 707,72 €
Preis: 707,72 €


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