Antibodies

Mouse Anti-FLAG Clone 1E6 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Mouse
Menge 100 ug
Host Mouse
ArtNr CS-CMAB1003
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
WB, IP
Description
The FLAG fusion tag consists of eight amino acids (DYKDDDDK) including an Enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of metal cations, preferably Ca(+2). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAG peptide. It is useful in the study of protein-protein interactions, cell ultrastructure, and protein localization. The FLAG peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
Formulation
Liquid in PBS + 50% Glycerol
Storage and Stability
Store at -20C. Avoid repeated freeze-thaw cycles.
Concentration
0.5 mg/mL Host: Mouse
Application Notes
Recommended dilution for WB: 0.5 mg/mLThe optimal concentration should be determined by the user for each specific application.

Arbeitsanleitung

CS-CMAB1003.pdf

Menge: 100 ug
Lieferbar: Out of stock
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