Rabbit Anti-Mouse Endoglin/CD105 pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Mouse
Isotype IgG
Applikationen ELISA
Menge 200 ug
ArtNr CS-CPC407B
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
ELISA: Use at 1-15 ug/ml. Western Analysis: Use at a concentration of 1-2 ug/ml with the appropriate secondary reagents. Immunohistochemistry: Not determined as of yet. Optimal dilutions should be determined by each laboratory for each application.
Description
Produced from sera of rabbits immunized with highly pure recombinant mouse soluble CD105/Endoglin produced in insect cells. The recombinant soluble CD105/Endoglin consists of amino acid 26 (Glu) to 581 (Gly) and is fused to a C-terminal His-tag (6xHis). Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating haematopoietic stem cells.
Formulation
Lyophilized rabbit IgG
Purification
Protein-A Chromatography (+his tag depleted)
Reconstitution
The lyophilized IgG is stable at 4C. for at least one month and for greater than a year when kept at -20C. When reconstituted in sterile water/PBS to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4C. Avoid repeated freeze-thaw cycles.
Menge: 200 ug
Lieferbar: In stock
Listenpreis: 494,49 €
Preis: 494,49 €
lieferbar

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