Human Granzyme B ELISPOT Kit w/ plates

Hersteller Cell Sciences
Typ Eli-Spot
Specific against Human
Menge 2 Plates
ArtNr CS-CKH007A
eClass 6.1 32160605
eClass 9.0 32160605
The ELISPOT (Enzyme-linked Immunospot) assay has been designed to identify and enumerate individual cells releasing specific proteins in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC). Cells are incubated in the wells of the ELISPOT plate precoated with a high-affinity monoclonal antibody to which the effector molecule, produced during incubation, will bind. Subsequently, cells are washed away. Areas in which the molecule has been trapped are detected with a combination of biotinylated detection antibodies and Streptavidin-horseradish peroxidase (Streptavidin-HRP). The last step in the assay is the addition of AEC (3-amino-9-ethylcarbazole) yielding a colored zone ('spot'). This zone reveals the site of effector molecule secretion.
Menge: 2 Plates
Lieferbar: In stock
Listenpreis: 1.011,03 €
Preis: 1.011,03 €


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