Human IL-4 ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CK405
Eclass 6.1 32160605
Eclass 9.0 32160605
IL-4 is a protein of 129 amino acids that is synthesized as a precursor containing a hydrophobic secretory signal sequence. IL-4 is glycosylated at two arginine residues (positions 38 and 105) and contains six cysteine residues involved in disulfide bond formation. The disulfide bonds are essential for biological activity. Some glycosylation variants of IL-4 have been described that differ in their biological activities. IL-4 enhancesexpression of class II MHC antigens on B-cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T-cells. IL-4 inhibits cell activation of NK-cells induced by IL-2. IL-4 stimulates the proliferation of thymocytes with the marker spectrum CD4 (-) CD8 (-), CD4 (+) CD8 (-), CD4 (-) CD8(+).The CellSciences Human IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-4 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human IL-4 coated on a 96-well plate. Standards and samples are pipettedinto the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 715,07 €
Preis: 715,07 €

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