Hersteller Cell Sciences
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CK412
Eclass 6.1 32160605
Eclass 9.0 32160605
Chemokine (C-C motif) Ligand 8, also known as Monocyte Chemotactic Protein 2 (MCP2), belongs to a family of secreted proteins involved in immunoregulatory and inflammatory processes structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. CCL8/MCP2 displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation CCL8/MCP2 may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. CCL8/MCP2 is found in the conditioned medium of human osteosarcoma cell line MG-63 that also secretes some other chemotactic factors including IL8 and CCL2/MCP1. CCL8/MCP2 is related closely to CCL2/MCP1 and CCL7/MCP3. Intradermal injection into rabbits causes the selective recruitment of monocytes at the site of injection. CCL8/MCP2 does not possess suppressive activity against immature subsets of myeloid progenitors stimulated to proliferate by multiple growth factors. The Human CCL8/MCP2 ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL8/MCP2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human CCL8/MCP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL8/MCP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human CCL8/MCP2 antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL8/MCP2 bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zip seal.
The minimum detectable dose of CCL8/MCP2 is typically less than 1.5 pg/mL.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 693,01 €
Preis: 693,01 €

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