Hersteller Cell Sciences
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CK417
Eclass 6.1 32160605
Eclass 9.0 32160605
The biological activities of the proteases are subject to a complex regulation also involving specific inhibitors, which are called TIMP (Tissue inhibitor of metalloproteinases). TIMP-1 is a protein of 28 kDa. The disruption of the TIMP-1gene in pluripotent embryonic stem cells can increase the invasive properties of these cells in an in vitro assay.The Cell Sciences Human TIMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIMP-1 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human TIMP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman TIMP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 693,01 €
Preis: 693,01 €

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Salesmanager: Arne Pelz