Human TNF-beta ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CK419
eClass 6.1 32160605
eClass 9.0 32160605
Human TNF-beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human TNF-beta in serum, plasma, cell culture supernatants and urine.This assay employs an antibody specific for human TNF-beta coated on a 96-well plate. Standards and samples arepipetted into the wells and TNF-beta present in a sample is bound to the wells by the immobilized antibody. The wellsare washed and biotinylated anti-human TNF-beta antibody is added. After washing away unbound biotinylatedantibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solutionis added to the wells and color develops in proportion to the amount of TNF-beta bound. The Stop Solution changesthe color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: Out of stock
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