Human VEGF ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH115
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Vascular Endothelial Growth Factor (VEGF) is also called VEGF-A, following the identification of several VEGF-related factors (VEGF-B through E). VEGF significantly influence vascular permeability and is a strong angiogenic protein and probably plays a role in neovascularisation under physiological conditions. VEGF plays a role in the development and function of primate follicles and the ovarian corpus luteum, supporting the proliferation of blood vessels. The differentiation of: adipocytes, pheochromocytomas, and myocytes is accompanied by the controlled expression of VEGF. It has been demonstrated that inhibition of VEGF activity by treatment with a monoclonal antibody specific for VEGF can suppress tumor growth, In vivo, . The Human VEGF ELISA Kit is an, In vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human VEGF antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of VEGF is typically less than 20 pg/mL.

Arbeitsanleitung

CS-CKH115.pdf

Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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