Human IL-1 alpha ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH116
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Description
Monocytes are the main source of secreted Interleukin-1 (IL1). They express predominantly IL1-beta (IL1B) while Human keratinocytes express large amounts of IL1-alpha (IL1A). IL1 is produced also by activated macrophages from different sources (alveolar macrophages, Kupffer cells, adherent spleen and peritoneal macrophages) and also by peripheral neutrophil granulocytes. IL1-alpha and IL1-beta are biologically more or less equivalent pleiotropic factors that act locally and also systemically. The main biological activity of IL1 is the stimulation of T-helper cells, which are induced to secrete IL2 and to express IL2 Receptors. The Human IL1-alpha ELISA Kit is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1-alpha in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human IL1-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human IL1-alpha antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1-alpha bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of IL1A is typically less than 2 pg/ml.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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