Human CD40 Ligand ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH128
eClass 6.1 32160605
eClass 9.0 32160605
CD40L is expressed predominantly on activated CD4+T lymphocytes, and also found in other types of cells, like NK cells, mastcells, basophils and eosinophils. CD40 is expressed on all B-lymphocytes during various stages of development, activated T-cellsand monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. CD40L mediates a range ofactivities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Igsecretion and memory generation. CD40 ligation has been shown to induce cell death by apoptosis in transformed cells ofmesenchymal and epithelial origin.The Cell Sciences Human CD40L ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human CD40L in serum, plasma (EDTA or heparin), cell culture supernatants and urine.This assay employs an antibody specific for human CD40L coated on a 96-well plate. Standards and samples are pipetted into thewells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylatedanti-human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipettedto the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to theamount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 786,58 €
Preis: 786,58 €


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