Human Eotaxin-2 ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH131
eClass 6.1 32160605
eClass 9.0 32160605
Eotaxin is composed of 73 amino acids and has been found in the bronchoalveolar lavage of guinea pigs used as a model ofallergic Inflammation. The factor belongs to the platelet factor-4 family of Chemokines. It exhibits homology of 53 percent withhuman MCP-1, 44 percent with guinea pig MCP-1, 31 percent with human MIP-1-alpha, and 26 percent with human RANTES.Eotaxin-2 induces chemotaxis of eosinophils and basophils, and the release of histamine and leukotriene C-4 from basophilsprimed with IL3. Eotaxin-2 activates chemotaxis-related events such as actin polymerization, and the respiratory burst almost asefficiently as Eotaxin.The Cell Sciences Human Eotaxin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linkedimmunosorbent assay for the quantitative measurement of human Eotaxin-2 in serum, plasma, cell culture supernatants and urine.This assay employs an antibody specific for human Eotaxin-2 coated on a 96-well plate. Standards and samples are pipetted intothe wells and Eotaxin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed andbiotinylated anti-human Eotaxin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugatedstreptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color developsin proportion to the amount of Eotaxin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity ofthe color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 786,58 €
Preis: 786,58 €


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