Human FGF4 ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH135
eClass 6.1 32160605
eClass 9.0 32160605
FGF (Fibroblast growth factors) constitutes a family of related 16-18 kDa proteins controlling normal growth and differentiation ofmesenchymal, epithelial, and neuroectodermal cell types. FGF-4 has potent transforming potential, apparently through an autocrinemechanism of action, and it is a potent angiogenic factor. It has also been extensively studied for its effects in embryogenesisThe Cell Sciences Human FGF-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human FGF-4 in serum, plasma, cell culture supernatants and urine. This assayemploys an antibody specific for human FGF-4 coated on a 96-well plate. Standards and samples are pipetted into the wells andFGF-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihumanFGF-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells.The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: Out of stock
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