Human GITR ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH141
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Description
GITR (glucocorticoid induced tumor necrosis factor receptor family related gene) is a type-1 transmembrane protein of 228 aminoacids belonging to the TNF and NGF receptor family of proteins. GITR is expressed in normal T-lymphocytes from thymus, spleen.Constitutive expression of a transfected GITR gene induces resistance to apoptosis induced by anti CD3 monoclonal antibodies.The human homolog of GITR is expressed in lymph node and peripheral blood leukocytes. Its expression is up-regulated in humanperipheral mononuclear cells mainly after stimulation with antibodies against CD3 and CD28.The Cell Sciences Human GITR ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human GITR in serum, plasma, cell culture supernatants and urine. This assay employsan antibody specific for human GITR coated on a 96-well plate. Standards and samples are pipetted into the wells and GITRpresent in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanGITR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells.The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITRbound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 786,58 €
Preis: 786,58 €
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