Human IGFBP2 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH149
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Introduction: IGFBPs (Insulin-like growth factor binding proteins) are found in various body fluids such as blood serum and amniotic fluid. They are synthesized in the liver and are also produced by various tumor cell lines and cell types. IGFBP-2 is observed mainly in brain, showing complex patterns of gene expression during postnatal brain development. Elevated levels of IGFBP-2 have been observed in the serum of prostate cancer patients. IGFBP-2 expression does not depend on Growth hormone. Assay Principle: The Human IGFBP-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an, in vitro, assay for the quantitative measurement of Human IGFBP-2 in serum, plasma, cell culture supernatants, and urine. This assay employs an antibody specific for human IGFBP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage and Stability
May be stored for 6 months at 2-4C. After reconstitution, store Standard (recombinant protein) at -80C. Opened Microplate Wells and reagents may be stored for 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge of zip-seal. The kit can be used within 1 year if the whole kit is stored at -20C. Avoid repeated freeze-thaw cycles.
Range
8.23 - 6, 000 pg/ml
Sensitivity
The minimum detectable dose of IGFBP-2 is typically less than 20 pg/ml.
Cross-Reactivity
This ELISA kit shows no cross-reactivity with any of the cytokines tested (human BDNF, BLC, ENA-78, FGF-4, IL-1a, IL-1a, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-a, IGFBP-1, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1a, MIP-1 a, MIP-1ä, PARC, PDGF, RANTES, SCF, TARC, TGF-a, TIMP-1, TIMP-2, TNF-a, TNF-a, TPO, VEGF).
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 715,07 €
Preis: 715,07 €
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