Human IL-17 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH157
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Interleukin 17 (IL17) enhances expression of the Intracellular Adhesion Molecule 1 (ICAM1) in human fibroblasts. Human IL17 also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL6, IL8, and G-CSF and prostaglandin E2. It functions as a mediator of angiogenesis that stimulates vascular endothelial cell migration and cord formation and regulates production of a variety of growth factors promoting angiogenesis (Numasaki et al). Mouse, Rat, and Human IL17 can induce IL6 secretion in Mouse stromal cells, indicating that all homologs can recognize the Mouse receptor. The Human IL17 ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL17 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human IL17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human IL17 antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL17 bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of IL17 is typically less than 10 pg/mL.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 715,07 €
Preis: 715,07 €
lieferbar

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Arbeitsanleitung

CS-CKH157.pdf