Human MIP-3 alpha/CCL20 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH171
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Chemokine (C-C motif) Ligand 20 (CCL20), also known as Macrophage Inflammatory Protein 3 alpha (MIP3A) is a small cytokine belonging to the C-C motif chemokine family. CCL20/MIP3A is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the surrounding epithelial cells. CCL20/MIP3A elicits its effects by binding and activating the receptor CCR6. Gene expression of CCL20/MIP3A can be induced by microbial factors such as LPS, and cytokines such as TNF and IFN gamma, while IL10 can down-regulate CCL20/MIP3A expression. CCL20/MIP3A is expressed in several tissues with highest levels in peripheral blood lymphocytes, lymph nodes, liver, appendix, and fetal lung. It plays a critical role in the regulation of dendritic cell trafficking, and the recruitment and activation of T-cells. The Human CCL20/MIP3A ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL20/MIP3A in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human CCL20/MIP3A coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL20/MIP3A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human CCL20/MIP3A antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL20/MIP3A bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of CCL20/MIP3A is typically less than 1.5 pg/mL.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 693,01 €
Preis: 693,01 €
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Arbeitsanleitung

CS-CKH171.pdf