Human NAP2/PPBP ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH178
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
Pro-Platelet Basic Protein (PPBP) is a Platelet-Derived Growth Factor belonging to the family of chemotactic cytokines. PPBP is also known as Chemokine (C-X-C motif) Ligand 7 (CXCL7) and Neutrophil-Activating Protein 2 (NAP2). It is induced in human monocytes by treatment with bacterial endotoxins. It stimulates the release of elastase from neutrophils and shows some of the activities of IL8. PPBP/NAP2 interacts with the NAP1 receptor and binds to one type of receptors that also binds IL8 and related factors. PPBP/NAP2 has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. PPBP/NAP2 also stimulates the formation and secretion of Plasminogen Activator by synovial cells. The Human PPBP/NAP2 ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human PPBP/NAP2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for PPBP/NAP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and PPBP/NAP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human PPBP/NAP2 antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PPBP/NAP2 bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
Sensitivity
The minimum detectable dose of PPBP/NAP2 is typically less than 8.5 pg/mL.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 693,01 €
Preis: 693,01 €
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Arbeitsanleitung

CS-CKH178.pdf