Human RANTES ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH183
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Description
RANTES is a protein, which belongs to the family of chemotactic cytokines. It is produced by circulating T-cells and T-cell clones in culture. RANTES activates human basophils from some select basophil donors and causes the release of histamines. It can induce the proliferation and activation of killer cells known as CHAK. RANTES is expressed by human synovial fibroblasts and may participate, therefore, in the ongoing inflammatory process in rheumatoid arthritis. The Cell Sciences Human RANTES ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RANTES in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Arbeitsanleitung

CS-CKH183.pdf

Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
lieferbar

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