Human CXCL12/SDF-1 alpha ELISA Kit

Hersteller Cell Sciences
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH184
eClass 6.1 32160605
eClass 9.0 32160605
Chemokine (C-X-C motif) Ligand 12, also known as SDF-1 (Stromal Cell-derived Factor-1) is a recently discovered protein belonging to the alpha chemokine (CXC) family of cytokines. SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a human bone-marrow stromal cell line. CXCL12/SDF-1 alpha has chemotactic activity on resting T-lymphocytes and monocytes. SDF-1 alpha and SDF-1 beta are small cytokines that belong to the Intercrine family, members of which activate leukocytes and are often induced by pro-inflammatory stimuli such as Lipopolysaccharide (LPS), Tumor Necrosis Factor (TNF), or Interleukin-1 (IL1). The Human CXCL12/SDF-1 alpha ELISA is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL12/SDF-1 alpha in plasma, cell culture supernatants and urine. This assay employs an antibody specific for CXCL12/SDF-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL12/SDF-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human CXCL12/SDF-1 alpha antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL12/SDF-1 alpha bound.
Storage and Stability
May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-4C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.
The minimum detectable dose of CXCL12 is typically less than 3.0 pg/mL.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €


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