Human ICAM1/CD54 ELISA Kit

Hersteller Cell Sciences
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 Plate
ArtNr CS-CKH186
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Description
sICAM-1 (soluble Intercellular Adhesion Molecular-1) has been reported in serum, cerebrospinal fluid and bronchoalveolar lavage.ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells. ICAM-1 is a ligand for LFA-1 (CD11a/CD18) andMac-1 (CD11b/CD18). Its expression is up-regulated upon stimulation by inflammatory mediators such as cytokines and LPS.The Cell Sciences Human sICAM-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linkedimmunosorbent assay for the quantitative measurement of human soluble ICAM-1 in serum, plasma, cell culture supernatants andurine. This assay employs an antibody specific for human sICAM-1 coated on a 96-well plate. Standards and samples are pipettedinto the wells and sICAM-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed andbiotinylated anti-human sICAM-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugatedstreptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color developsin proportion to the amount of sICAM-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Menge: 1 Plate
Lieferbar: In stock
Listenpreis: 762,31 €
Preis: 762,31 €
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